XPG protein has a structure-specific endonuclease activity
نویسندگان
چکیده
منابع مشابه
Design, activity, and structure of a highly specific artificial endonuclease.
We have generated an artificial highly specific endonuclease by fusing domains of homing endonucleases I-DmoI and I-CreI and creating a new 1400 A(2) protein interface between these domains. Protein engineering was accomplished by combining computational redesign and an in vivo protein-folding screen. The resulting enzyme, E-DreI (Engineered I-DmoI/I-CreI), binds a long chimeric DNA target site...
متن کاملThe founding members of xeroderma pigmentosum group G produce XPG protein with severely impaired endonuclease activity.
Of the eight human genes implicated in xeroderma pigmentosum, defects in XPG produce some of the most clinically diverse symptoms. These range from mild freckling to severe skeletal and neurologic abnormalities characteristic of Cockayne syndrome. Mildly affected xeroderma pigmentosum group G patients have diminished XPG endonuclease activity in nucleotide excision repair, whereas severely affe...
متن کاملThe replication initiator protein of plasmid pT181 has sequence-specific endonuclease and topoisomerase-like activities.
Initiation of pT181 DNA replication specifically requires the plasmid-encoded RepC protein. Here we demonstrate that highly purified RepC protein has sequence-specific endonuclease and topoisomerase-like activities. A maximum sequence of 127 base pairs containing the pT181 origin of replication is required for nicking-closing by RepC protein. RepC introduces a single strand break within the pT1...
متن کاملPso2 (SNM1) is a DNA structure-specific endonuclease
Many types of DNA structures are generated in response to DNA damage, repair and recombination that require processing via specialized nucleases. DNA hairpins represent one such class of structures formed during V(D)J recombination, palindrome extrusion, DNA transposition and some types of double-strand breaks. Here we present biochemical and genetic evidence to suggest that Pso2 is a robust DN...
متن کاملSequence- and structure-specific RNA processing by a CRISPR endonuclease.
Many bacteria and archaea contain clustered regularly interspaced short palindromic repeats (CRISPRs) that confer resistance to invasive genetic elements. Central to this immune system is the production of CRISPR-derived RNAs (crRNAs) after transcription of the CRISPR locus. Here, we identify the endoribonuclease (Csy4) responsible for CRISPR transcript (pre-crRNA) processing in Pseudomonas aer...
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ژورنال
عنوان ژورنال: Mutation Research Letters
سال: 1995
ISSN: 0165-7992
DOI: 10.1016/0165-7992(95)90070-5